Holland D J, Bliss K J, Allen C D, Gilbert G L
Centre for Infectious Diseases and Microbiology, Westmead Hospital, Australia.
Pathology. 1995 Jan;27(1):91-6. doi: 10.1080/00313029500169562.
Two different commercially available urine dipsticks were evaluated to determine their usefulness as a screening test for the detection of non-infected urine specimens. The reactions of both dipstick strips were read visually and, in addition, one was read by semi-automated reflectance photometry. 2928 consecutive routine urine specimens received by the microbiology laboratory during normal working hours underwent testing by the conventional method of microscopy and culture and the results were compared with those obtained by dipstick testing. There were poor correlations between microscopy for red and white blood cells and dipstick results for blood and leucocyte esterase respectively. The dipsticks had a low positive predictive value (PPV) in identifying infected urines. In contrast, dipsticks had a very high negative predictive value (NPV) of 98.6-99.5% and correctly identified a significant proportion (about 1/3) of non-infected urines as reported by the conventional method. Introduction of dipstick testing of urine specimens in our hospital has led to significant time saving as dipstick-negative urines are not processed further (except for specific clinical indications) and there has also been a significant decrease (25%) in the number of specimens submitted to the laboratory. Further efficiencies may be obtainable with increased automation.
对两种不同的市售尿试纸进行了评估，以确定它们作为检测未感染尿液标本的筛查试验的效用。两种试纸条的反应均通过肉眼读取，此外，其中一种还通过半自动反射光度法读取。微生物实验室在正常工作时间收到的2928份连续常规尿液标本采用传统的显微镜检查和培养方法进行检测，并将结果与尿试纸检测结果进行比较。红细胞和白细胞显微镜检查结果与尿试纸血液和白细胞酯酶检测结果之间的相关性较差。尿试纸在识别感染尿液方面的阳性预测值（PPV）较低。相比之下，尿试纸的阴性预测值（NPV）非常高，为98.6-99.5%，并且能够正确识别出相当比例（约1/3）的未感染尿液，这与传统方法报告的结果一致。在我院引入尿试纸检测尿液标本可显著节省时间，因为尿试纸检测为阴性的尿液不再进一步处理（特定临床指征除外），提交给实验室的标本数量也显著减少（25%）。随着自动化程度的提高，可能会进一步提高效率。
