一种A-DNA三联体密码：预测A-DNA和B-DNA的热力学规则。
An A-DNA triplet code: thermodynamic rules for predicting A- and B-DNA.
作者信息
Basham B, Schroth G P, Ho P S
机构信息
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6464-8. doi: 10.1073/pnas.92.14.6464.
Abstract
The ability to predict macromolecular conformations from sequence and thermodynamic principles has long been coveted but generally has not been achieved. We show that differences in the hydration of DNA surfaces can be used to distinguish between sequences that form A- and B-DNA. From this, a "triplet code" of A-DNA propensities was derived as energetic rules for predicting A-DNA formation. This code correctly predicted > 90% of A- and B-DNA sequences in crystals and correlates with A-DNA formation in solution. Thus, with our previous studies on Z-DNA, we now have a single method to predict the relative stability of sequences in the three standard DNA duplex conformations.
摘要
长期以来，人们一直渴望能够根据序列和热力学原理预测大分子构象，但通常尚未实现。我们表明，DNA表面水合作用的差异可用于区分形成A-DNA和B-DNA的序列。据此，推导出了A-DNA倾向的“三联体密码”，作为预测A-DNA形成的能量规则。该密码正确预测了晶体中>90%的A-DNA和B-DNA序列，并与溶液中A-DNA的形成相关。因此，结合我们之前对Z-DNA的研究，我们现在有一种单一方法来预测三种标准DNA双链构象中序列的相对稳定性。
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