Holk K, Lykkeboe G
Department of Zoophysiology, Aarhus University, Denmark.
Respir Physiol. 1995 Apr;100(1):55-62. doi: 10.1016/0034-5687(94)00118-j.
Carp and trout blood maintained at low constant oxygen and carbon dioxide tensions was beta-stimulated. This activated the Na+/H(+)-exchanger of the red cell membrane, leading to increases in red cell pH (pHi) and cell water content, the latter resulting in dilution of hemoglobin and organic phosphates. The increase in pHi was rapid and maintained throughout the experimental period, the trout red cells showing the largest increase. Likewise swelling of the red cells was larger in trout than in carp blood. As a consequence of beta-stimulation the oxygen affinity of the blood increased. In trout the intracellular Bohr factor of unstimulated blood combined with the pHi increase upon stimulation could account for 85% of the increase in oxygen affinity, whereas it only covered 65% of the increase in carp blood. We therefore conclude that blood oxygen affinity is dependent on the red cell hemoglobin concentration in both species, the effect being more marked in carp.
将鲤鱼和鳟鱼的血液维持在低恒定氧分压和二氧化碳分压条件下，会受到β刺激。这激活了红细胞膜的Na⁺/H⁺交换体，导致红细胞内pH值（pHi）升高和细胞含水量增加，后者导致血红蛋白和有机磷酸盐稀释。pHi的升高迅速且在整个实验期间保持，鳟鱼红细胞的升高幅度最大。同样，鳟鱼红细胞的肿胀比鲤鱼血液中的更大。β刺激的结果是血液的氧亲和力增加。在鳟鱼中，未刺激血液的细胞内玻尔因子与刺激后pHi的升高可解释氧亲和力增加的85%，而在鲤鱼血液中仅占增加量的65%。因此，我们得出结论，两种鱼类的血液氧亲和力均取决于红细胞血红蛋白浓度，在鲤鱼中这种影响更为显著。
