Brauner C J, Gilmour K M, Perry S F
Department of Zoophysiology, Aarhus University, Denmark.
Respir Physiol. 1996 Oct;106(1):65-70. doi: 10.1016/0034-5687(96)00061-8.
CO2 excretion in trout is dependent upon the Haldane effect and the release of protons (Bohr protons) from haemoglobin during oxygenation of the blood. Oxygenation of whole blood from trout in vitro, resulted in a non-linear release of Bohr protons over the haemoglobin-oxygen (Hb-O2) equilibrium curve, where the majority of Bohr protons were released between 60 and 100% of Hb oxygen saturation (SO2). Oxygenation of the blood over this region of the Hb-O2 equilibrium curve elevated the HCO3- flux rate across the HCO3-/CI- exchanger on the red cell membrane by about 30% during CO2 excretion in vitro. Oxygenation of the Hb between 0 and 60% SO2 did not elevate CO2 excretion rate in vitro. These data indicate that utilization of different regions of the Hb-O2 dissociation curve in trout in vivo will influence CO2 excretion by altering the rate of HCO3- entry into the red cell during gill blood transit.
虹鳟鱼的二氧化碳排泄取决于哈代效应以及血液氧合过程中血红蛋白释放质子（波尔质子）。体外对虹鳟鱼的全血进行氧合，导致在血红蛋白-氧气（Hb-O₂）平衡曲线上波尔质子呈非线性释放，其中大部分波尔质子在血红蛋白氧饱和度（SO₂）的60%至100%之间释放。在体外二氧化碳排泄过程中，在Hb-O₂平衡曲线的这一区域对血液进行氧合，可使红细胞膜上HCO₃⁻/Cl⁻交换体的HCO₃⁻通量率提高约30%。在体外，SO₂为0至60%时血红蛋白的氧合不会提高二氧化碳排泄率。这些数据表明，虹鳟鱼体内利用Hb-O₂解离曲线的不同区域将通过改变鳃血转运过程中HCO₃⁻进入红细胞的速率来影响二氧化碳排泄。
