He Y, Revel M, Loty B
Höpital Cochin, Service de Chirurgie Orthopédique, Paris, France.
Spine (Phila Pa 1976). 1995 Mar 1;20(5):557-63; discussion 579-80. doi: 10.1097/00007632-199503010-00010.
A quantitative model of peridural post-laminectomy fibrosis in rats was designed in this study. Concurrently, the effects of a nonsteroidal anti-inflammatory drug (ketoprofen) were evaluated.
To quantify the amount of fibrotic tissue, the extent of adhesion to the dura mater, and the cell nature and density for drug trials requiring large series of animals.
Most of the previous analyses of experimental post-laminectomy fibrosis were qualitative in nature. Only one quantitative analysis was reported in the rabbit, and the rat has seldom been used. The effects of nonsteroidal anti-inflammatory drugs on post-laminectomy scar formation have never been evaluated.
L5 laminectomies were performed in 32 rats. The treated group (16 rats) received a systemic injection of ketoprofen (5 mg/kg, once daily from the day before the operation to the seventh postoperative day), the other 16 rats constituted a control group. The post-laminectomy scar formation was evaluated on postoperative days 8, 15, 30, and 90 using a semiautomatic image analysis system.
The course of post-laminectomy scar formation was similar to that described in larger animals. There was a good correlation among the measurements of fibrous tissue area obtained by 2 independent observers. The mean amount of peridural scar tissue was significantly smaller in the treated group than in the control group. The extent of adherence to the dura mater and the density of fibroblasts and fibrocytes was not different between the two groups. The density of inflammatory cells was significantly less in the treated group than in the control group only at day 8.
Quantitative evaluation of post-laminectomy fibrosis can easily be performed in rats and is reproducible. This model could allow trials with drugs administered locally or systemically as preventive treatment of post-laminectomy scar formation. Nonsteroidal anti-inflammatory drugs could decrease the amount of fibrotic tissue.
本研究设计了大鼠椎板切除术后硬膜外纤维化的定量模型。同时，评估了一种非甾体抗炎药（酮洛芬）的效果。
为需要大量动物的药物试验量化纤维化组织的数量、与硬脑膜的粘连程度以及细胞性质和密度。
以往大多数对实验性椎板切除术后纤维化的分析本质上都是定性的。仅在兔子身上报道过一项定量分析，而大鼠很少被使用。非甾体抗炎药对椎板切除术后瘢痕形成的影响从未被评估过。
对32只大鼠进行L5椎板切除术。治疗组（16只大鼠）接受酮洛芬全身注射（5毫克/千克，从手术前一天至术后第七天每天一次），另外16只大鼠作为对照组。在术后第8、15、30和90天使用半自动图像分析系统评估椎板切除术后瘢痕形成情况。
椎板切除术后瘢痕形成过程与在大型动物中描述的相似。两名独立观察者获得的纤维组织面积测量值之间具有良好的相关性。治疗组硬膜外瘢痕组织的平均数量明显少于对照组。两组之间与硬脑膜的粘连程度以及成纤维细胞和纤维细胞的密度没有差异。仅在第8天，治疗组的炎症细胞密度明显低于对照组。
在大鼠中可以轻松进行椎板切除术后纤维化的定量评估，并且具有可重复性。该模型可用于局部或全身给药作为椎板切除术后瘢痕形成预防性治疗的药物试验。非甾体抗炎药可减少纤维化组织的数量。
