中国仓鼠卵巢细胞系CHO-K1中编码葡萄糖磷酸异构酶和磷酸甘油酸激酶的cDNA的特性分析以及CHO-K1糖酵解缺陷变体R1.1.7中的缺陷鉴定。
Characterization of cDNAs coding for glucose phosphate isomerase and phosphoglycerate kinase in Chinese hamster ovary cell line CHO-K1 and identification of defects in R1.1.7, a glycolysis-deficient variant of CHO-K1.
作者信息
Hassan-Walker A F, Morgan M J, Faik P
机构信息
Wellcome Laboratory For Molecular Genetics, Division of Biochemistry and Molecular Biology, UMDS, London, UK.
出版信息
Somat Cell Mol Genet. 1995 Jan;21(1):75-81. doi: 10.1007/BF02255824.
PMID:7604358
Abstract
Full-length cDNAs for glucose phosphate isomerase (GPI) and phosphoglycerate kinase (PGK) of the Chinese hamster ovary cell line CHO-K1 have been characterized using RT-PCR and cycle sequencing of the PCR-amplified templates. Mutations in both genes have been identified in a glycolysis-deficient Chinese hamster ovary cell line, R1.1.7, derived from CHO-K1 cells.
摘要
利用逆转录聚合酶链反应（RT-PCR）以及对聚合酶链反应（PCR）扩增模板进行循环测序，对中国仓鼠卵巢细胞系CHO-K1中的磷酸葡萄糖异构酶（GPI）和磷酸甘油酸激酶（PGK）的全长互补DNA（cDNA）进行了表征。在源自CHO-K1细胞的糖酵解缺陷型中国仓鼠卵巢细胞系R1.1.7中，已鉴定出这两个基因的突变。
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