Pellestor F, Girardet A, Lefort G, Andréo B, Charlieu J P
CNRS UPR 9008, Montpellier, France.
Am J Med Genet. 1995 May 8;56(4):393-7. doi: 10.1002/ajmg.1320560409.
The "PRimed IN Situ labeling" (PRINS) method is an interesting alternative to in situ hybridization for chromosomal detection. In this procedure, chromosome labeling is performed by in situ annealing of specific oligonucleotide primers, followed by primer elongation by a Taq polymerase in the presence of labeled nucleotides. Using this process, we have developed a simple and semi-automatic method for rapid in situ detection of human chromosome 21. The reaction was performed on a programmable temperature cycler, with a chromosome 21 specific oligonucleotide primer. Different samples of normal and trisomic lymphocytes and amniotic fluid cells were used for testing the method. Specific labeling of chromosome 21 was obtained in both metaphases and interphase nuclei in a 1 hour reaction. The use of oligonucleotide primer for in situ labeling overcomes the need for complex preparations of specific DNA probes. The present results demonstrate that PRINS may be a simple and reliable technique for rapidly detecting aneuploidies.
“原位引物标记法”（PRINS）是一种用于染色体检测的有趣的原位杂交替代方法。在此过程中，通过特定寡核苷酸引物的原位退火进行染色体标记，随后在标记核苷酸存在的情况下由Taq聚合酶进行引物延伸。利用这一过程，我们开发了一种简单的半自动方法，用于快速原位检测人类21号染色体。反应在可编程温度循环仪上进行，使用21号染色体特异性寡核苷酸引物。使用正常和三体淋巴细胞以及羊水细胞的不同样本对该方法进行测试。在1小时的反应中，在中期和间期核中均获得了21号染色体的特异性标记。使用寡核苷酸引物进行原位标记无需复杂制备特异性DNA探针。目前的结果表明，PRINS可能是一种快速检测非整倍体的简单可靠技术。
