[Rapid in situ detection of chromosome 21 by the PRINS technique].
作者信息
Pellestor F, Girardet A, Lefort G, Andréo B, Charlieu J P
机构信息
CNRS UPR 9008, Montpellier, France.
出版信息
Ann Genet. 1994;37(2):66-71.
PMID:7985980
Abstract
The PRINS technique has been used to rapidly detect chromosomes 21 in both metaphases and interphase nuclei. A specific labeling was obtained in preparations from lymphocytes and amniotic fluid cells. The method is based on annealing of specific oligonucleotide primers and subsequent primer extension by a Taq DNA polymerase. PRINS is an interesting alternative to in situ hybridization for cytogenetic diagnosis and physical mapping.
摘要
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