Brink P R, Kensler R W, Dewey M M
Am J Anat. 1979 Jan;154(1):11-26. doi: 10.1002/aja.1001540103.
The nexus of the anterior byssus retractor muscle (ABRM) of Mytilus has been observed with freeze-fracture techniques. Pits appear in the EF face and particles in the PF face, designating it as an A-type nexus. Under control conditions (lanthanum-free) the center-to-center spacing of pits and particles ranged from 14.0 nm to 15.5 nm. When lanthanum chloride (5 mM) was used in incubation media before fixation and glycerination, marked changes in the nexal membrane were observed. These changes involved close packing and long-range ordering of pits in the EF face, as shown by optical diffraction. In the presence of lanthanum the center-to-center spacing of the pits was 11.7 nm, while the particles remained at a spacing of 15.0 nm. From this we conclude that specific agents can alter the array of pits and particles in a nexus (gap junction).
利用冷冻断裂技术观察了贻贝前足丝牵缩肌（ABRM）的连接。在EF面上出现凹坑，在PF面上出现颗粒，将其确定为A型连接。在对照条件下（无镧），凹坑和颗粒的中心间距在14.0纳米至15.5纳米之间。在固定和甘油处理前，将氯化镧（5 mM）用于孵育培养基时，观察到连接膜有明显变化。这些变化包括EF面上凹坑的紧密堆积和长程有序排列，如光学衍射所示。在镧存在的情况下，凹坑的中心间距为11.7纳米，而颗粒的间距保持在15.0纳米。由此我们得出结论，特定试剂可以改变连接（间隙连接）中凹坑和颗粒的排列。
