Cortés A, Fonfría J, Vicente A, Varas A, Moreno J, Zapata A G
Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
Anat Rec. 1995 May;242(1):91-5. doi: 10.1002/ar.1092420112.
The so-called diffusely infiltrated lymphoid tissue of the chicken bursa of Fabricius was previously described as a T-dependent bursal area.
We have analyzed immunohistologically its postnatal development by using a battery of mAbs, most of them raised specifically to chicken T cells, in order to characterize phenotypically the nature of its cell components, appearance, development, and possible functional significance.
Our results demonstrate that this tissue represents poorly developed lymphoid aggregates, the cell content reminiscent of that found in other lymphoid tissues occurring throughout the avian gut. The first lymphoid elements appear in this bursal area only after hatching, growing rapidly to reach the adult condition in the second week of postnatal life. They consist mainly of T lymphocytes, including principally. CD4+ TcR alpha V beta 1+ cells, which form cell groups and CD8+ cells, and TcR gamma delta+ lymphocytes, which predominate in the subepithelial area and within the epithelium. MHC Class II molecule-expressing cells and IgM-, IgG-, and IgA-positive B lymphocytes also occupy the subepithelial region.
We propose that the bursal diffusely infiltrated lymphoid tissue of the chicken represents gut-associated lymphoid tissue involved in mounting immune responses to antigens that reach the bursal lumen via the cloaca.
鸡法氏囊中所谓的弥漫性浸润淋巴组织先前被描述为一个T细胞依赖的囊区。
我们通过使用一系列单克隆抗体对其出生后的发育进行了免疫组织学分析，其中大多数单克隆抗体是专门针对鸡T细胞产生的，以便从表型上表征其细胞成分的性质、外观、发育以及可能的功能意义。
我们的结果表明，该组织代表发育不良的淋巴聚集物，其细胞成分让人联想到在整个禽肠道中出现的其他淋巴组织中的细胞成分。第一批淋巴样细胞仅在孵化后出现在这个囊区，迅速生长，在出生后第二周达到成年状态。它们主要由T淋巴细胞组成，主要包括形成细胞群的CD4+TcRαVβ1+细胞和CD8+细胞，以及在上皮下区域和上皮内占主导地位的TcRγδ+淋巴细胞。表达MHC II类分子的细胞以及IgM、IgG和IgA阳性B淋巴细胞也占据上皮下区域。
我们提出，鸡囊弥漫性浸润淋巴组织代表与肠道相关的淋巴组织，参与对通过泄殖腔到达囊腔的抗原产生免疫反应。
