David J C, Fedecka-Bruner B, Vaigot P
Eur J Immunol. 1985 Jan;15(1):77-82. doi: 10.1002/eji.1830150115.
The activities of 8 S and 6 S DNA ligases have been studied in the chicken lymphoid cells of blood, spleen and bursa of Fabricius at different stages of development, from late embryonic life to about 3 months after hatching. These cells have been sorted with the fluorescence-activated cell sorter FACS II on the basis of size and T or B antigenicity (immunofluorescence). The light 6 S DNA ligase has been previously demonstrated to be associated to a late stage of differentiation of thymocytes. In the bursa, a unique form of 8 S DNA ligase is found during the whole period of observation. This form of enzyme remains in the B cells of the spleen until 3 weeks after hatching, but is never present in the blood B cells. As far as T cells are concerned, the light DNA ligase is present in the blood from 18-day embryonic life on. In the spleen T cells, on the contrary, this enzyme appears only 3 weeks after hatching. Before this stage, splenic T cells are devoid of any form of DNA ligase activity. These findings show biochemical differences in T and B lymphocytes colonizing the periphery, blood and spleen, and suggest, at least for the T cells at early stages, a heterogeneity in the degree of differentiation.
在从胚胎后期到孵化后约3个月的不同发育阶段，对鸡血、脾脏和法氏囊的淋巴样细胞中8S和6S DNA连接酶的活性进行了研究。这些细胞已根据大小以及T或B抗原性（免疫荧光），用荧光激活细胞分选仪FACS II进行了分选。先前已证明轻链6S DNA连接酶与胸腺细胞分化的后期相关。在法氏囊中，在整个观察期间发现了一种独特形式的8S DNA连接酶。这种酶形式在孵化后3周前一直存在于脾脏的B细胞中，但从未出现在血液B细胞中。就T细胞而言，从胚胎18天起血液中就存在轻链DNA连接酶。相反，在脾脏T细胞中，这种酶在孵化后3周才出现。在此阶段之前，脾脏T细胞没有任何形式的DNA连接酶活性。这些发现表明，在外周、血液和脾脏中的T淋巴细胞和B淋巴细胞存在生化差异，并且至少对于早期阶段的T细胞而言，提示了分化程度的异质性。
