Hillmann G, Hillmann B, Geurtsen W
Department of Conservative Dentistry and Periodontology, Dental School, Medical University Hannover, Germany.
Arch Oral Biol. 1995 Apr;40(4):353-9. doi: 10.1016/0003-9969(94)00136-y.
Interleukin-1 beta (Il-1 beta) is the predominant form of Il-1 produced by monocytes of the blood and by macrophages of various tissues. Human keratinocytes express both Il-1 alpha and Il-1 beta mRNA, but appear to produce mainly Il-1 alpha. The aim of this study was to determine the localization of interleukin-1 beta and interleukin-1 beta receptors in human gingival epithelium by different immunohistochemical methods. The alkaline phosphatase and the immunogold staining technique, as well as fluorescence microscopy, were used to investigate active participation of gingival epithelial cells in the development of periodontitis. Biopsies of human interdental papillae showed some activity of epithelial cells in the production of Il-1 beta. Single cells, clusters or larger areas of the sulcular and oral epithelium appeared to produce Il-1 beta at inflamed sites, and in these areas the normal epithelial structure was disturbed. Epithelial cells grown from the same biopsies appear able to express specific receptor molecules for Il-1 beta under normal culture conditions. It is concluded that gingival keratinocytes might be activated by inflammatory irritants and participate actively in the inflammatory processes.
白细胞介素 -1β（IL -1β）是血液中的单核细胞和各种组织中的巨噬细胞产生的主要IL -1形式。人角质形成细胞同时表达IL -1α和IL -1β mRNA，但似乎主要产生IL -1α。本研究的目的是通过不同的免疫组织化学方法确定白细胞介素 -1β和白细胞介素 -1β受体在人牙龈上皮中的定位。采用碱性磷酸酶和免疫金染色技术以及荧光显微镜来研究牙龈上皮细胞在牙周炎发展中的积极参与情况。人牙间乳头活检显示上皮细胞在产生IL -1β方面有一定活性。在炎症部位，龈沟上皮和口腔上皮的单个细胞、细胞簇或较大区域似乎能产生IL -1β，并且在这些区域正常上皮结构受到破坏。从相同活检组织培养的上皮细胞在正常培养条件下似乎能够表达IL -1β的特异性受体分子。得出的结论是牙龈角质形成细胞可能被炎症刺激物激活并积极参与炎症过程。
