Dixit U B, Kelly K M, Squier C A, Bardach J
Department of Pediatric Dentistry, University of Iowa, Iowa City 52242-1010, USA.
Cleft Palate Craniofac J. 1995 May;32(3):228-34. doi: 10.1597/1545-1569_1995_032_0228_piropd_2.3.co_2.
The influence of the periosteum on the regeneration of palatal bone was investigated in this study. Eighty, 8-week-old, purebred beagle dogs were assigned randomly to four groups: (1) unoperated dogs as a group control; dogs in which the mid-third of the palate was surgically removed and (2) left unrepaired (unrepaired controls); (3) repaired with mucosal flaps; (4) repaired with mucoperiosteal flaps. Five animals from each group were killed at 4, 6, 8, and 12 weeks after surgery and coronal sections examined under light microscopy. Among the animals with complete soft tissue healing, 8 of 12 dogs from Group 2, 11 of 20 from Group 3, and 10 of 19 from Group 4 showed complete bone regeneration. No significant differences were found overall in bone thickness and bone density measurements between Group 3 and Group 4. Histologically, a well-differentiated periosteum was present on the maxilla at 4 weeks, even in animals in which the periosteum had not been preserved in the original flap. These results suggest that maintaining the periosteum at surgical closure does not influence bone regeneration in beagles up to 12 weeks of age. We suggest that osteoprogenitor cells, migrating from the undisturbed local periosteum adjacent to the defect, were responsible for the new bone growth in our study.
本研究调查了骨膜对腭骨再生的影响。80只8周龄的纯种比格犬被随机分为四组：（1）未手术的犬作为组对照；（2）将腭中部手术切除且不修复的犬（未修复对照）；（3）用黏膜瓣修复的犬；（4）用黏骨膜瓣修复的犬。术后4、6、8和12周时，每组处死5只动物，取冠状切片进行光学显微镜检查。在软组织完全愈合的动物中，第2组12只犬中有8只、第3组20只中有11只、第4组19只中有10只显示完全骨再生。第3组和第4组之间在骨厚度和骨密度测量方面总体未发现显著差异。组织学上，术后4周时上颌骨存在分化良好的骨膜，即使在原始瓣中未保留骨膜的动物中也是如此。这些结果表明，手术闭合时保留骨膜对12周龄以内比格犬的骨再生没有影响。我们认为，在我们的研究中，从缺损附近未受干扰的局部骨膜迁移而来的骨祖细胞是新骨生长的原因。
