Fujioka M, Fujii T
Department of Plastic and Reconstructive Surgery, Nagasaki University of Medicine, Japan.
Cleft Palate Craniofac J. 1997 Jul;34(4):297-308. doi: 10.1597/1545-1569_1997_034_0297_mgfaio_2.3.co_2.
The implantation of atelocollagen matrix on the denuded surface of palatal bone following cleft palate repair has been used because it enhances wound healing. This study was performed to determine whether the beneficial effect of atelocollagen matrix implantation on the prevention of scar tissue contraction also inhibits the scar's interference with the growth of maxillary bone.
Fifty New Zealand White rabbits (aged 4 weeks) underwent palatal mucoperiosteal denudation, and etelocollagen matrix was implanted on the left palatal process. The opposite side was left open as a control.
Histopathologically, the implantation side exhibited early infiltration of mononuclear cells and fibroblasts, and better growth of connective tissue strands and epithelium. In addition, the formation of rate ridges were seen that were similar to the normal mucosa. The bone of the atelocollagen-implanted side was covered with regenerated periosteum-like layers, but that of the control side was lined by granulation tissue, suggesting the existence of continuous inflammation on the periosteal region. When the animals reached adulthood (aged 24 weeks), the areas of scars and palatal processes, palatal shelf width, molar teeth incline, and bone mineral contents were measured and compared between sides. The atelocollagen-applied scars showed less contraction, the area and width of atelocollagen-implanted palatal processes showed more satisfactory growth, and the dental arch deformity was suppressed in comparison with the control side.
Our results suggest that the use of atelocollagen matrix on the denuded bone surface following cleft palate repair decreases the scar's effect on maxillary growth.
腭裂修复术后在裸露的腭骨表面植入去端胶原蛋白基质，因其可促进伤口愈合。本研究旨在确定去端胶原蛋白基质植入对预防瘢痕组织收缩的有益作用是否也能抑制瘢痕对上颌骨生长的干扰。
50只4周龄的新西兰白兔接受腭黏骨膜剥脱术，在左侧腭突植入去端胶原蛋白基质。另一侧不做处理作为对照。
组织病理学检查显示，植入侧有单核细胞和成纤维细胞早期浸润，结缔组织束和上皮生长较好。此外，可见形成了与正常黏膜相似的嵴。植入去端胶原蛋白侧的骨表面覆盖有再生的类骨膜层，而对照侧则为肉芽组织，提示骨膜区域存在持续炎症。当动物成年（24周龄）时，测量并比较两侧瘢痕面积、腭突面积、腭架宽度、磨牙倾斜度和骨矿物质含量。与对照侧相比，应用去端胶原蛋白的瘢痕收缩较小，植入去端胶原蛋白的腭突面积和宽度生长更理想，牙弓畸形得到抑制。
我们的结果表明，腭裂修复术后在裸露的骨表面使用去端胶原蛋白基质可降低瘢痕对上颌生长的影响。
