Murase S
Department of Anatomy, School of Medicine, Keio University, Tokyo, Japan.
Neuroreport. 1995 Mar 27;6(5):777-81. doi: 10.1097/00001756-199503270-00018.
Molecular layers of the adult rat cerebellum, the inferior olivary neurones and climbing fibres which had been destroyed by 3-acetylpyridine (3-AP) and inferior cerebellar pedunculotomy, were examined using an antibody against corticotropin releasing factor (CRF) to visualize mossy fibres. Sprouting mossy fibres appeared in the molecular layer 2 days after 3-AP treatment. Morphologically, they showed no bundle formation, rosette-like swellings along their course, made ectopic synapses with interneurones, and had no association with arrested granule cells. The number of sprouting fibres gradually increased with time after 3-AP treatment. These findings indicate that mossy fibre sprouting is continuously induced in the climbing fibre-deficient cerebellum.
利用抗促肾上腺皮质激素释放因子（CRF）抗体来观察苔藓纤维，对成年大鼠小脑的分子层、被3-乙酰吡啶（3-AP）和小脑下脚切断术破坏的下橄榄核神经元及攀缘纤维进行了检查。在3-AP处理后2天，分子层出现了发芽的苔藓纤维。从形态学上看，它们没有形成束状，在其行程中有玫瑰花结样肿胀，与中间神经元形成异位突触，并且与静止的颗粒细胞没有关联。3-AP处理后，发芽纤维的数量随时间逐渐增加。这些发现表明，在攀缘纤维缺失的小脑中，苔藓纤维发芽是持续被诱导的。
