Kirpekar F, Nordhoff E, Kristiansen K, Roepstorff P, Hahner S, Hillenkamp F
Department of Molecular Biology, University of Odense, Denmark.
Rapid Commun Mass Spectrom. 1995;9(6):525-31. doi: 10.1002/rcm.1290090611.
Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
含有正常嘌呤核苷酸7-脱氮类似物的寡脱氧核苷酸已通过酶法和化学法合成。当进行基质辅助激光解吸/电离质谱（MALDI-MS）分析时，与其他相同的未修饰寡脱氧核苷酸相比，修饰后的样品具有更高的稳定性和更高的灵敏度。鉴于这些观察结果，本文提出了在正离子检测模式下MALDI-MS中寡脱氧核苷酸的碎片化模型。此外，还讨论了7-脱氮嘌呤核苷酸在DNA测序反应的MALDI-MS分析中的潜在用途。
