Reed K M, Bohlander S K, Phillips R B
Department of Biological Sciences, University of Wisconsin-Milwaukee 53201, USA.
Chromosome Res. 1995 Jun;3(4):221-6. doi: 10.1007/BF00713046.
Lake trout, Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.
湖鳟（Salvelinus namaycush）是少数具有形态学分化性染色体的鲑科鱼类之一。遗传分析表明，该物种的性别决定区域位于Y染色体的短臂上。对Y染色体的差异臂进行显微切割，并以序列非依赖方式扩增所得DNA。扩增的DNA用生物素标记作为荧光原位杂交（FISH）的探针。在Y和X染色体的特定区域均观察到强杂交信号。用Y探针进行FISH未鉴定出祖先四倍体基因组的同源染色体，表明该基因组区域已二倍体化。
