Pendás A M, Morán P, García-Vázquez E
Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Chromosoma. 1994 Apr;103(2):147-52. doi: 10.1007/BF00352324.
The major histone cluster (hisDNA) was mapped by fluorescent in situ hybridization (FISH) to mitotic chromosomes of Atlantic salmon, brown trout, and rainbow trout. The data reveal that in the three species hisDNA is tandemly repeated in a single locus. Southern blots of genomic DNA indicate that these clusters are representative of the vast majority of the histone genes in these species. Similar reiteration values were found among the three species. Genetic variability in the hisDNA was found only in brown trout for an EcoRI site.
通过荧光原位杂交（FISH）将主要组蛋白簇（hisDNA）定位到大西洋鲑、褐鳟和虹鳟的有丝分裂染色体上。数据显示，在这三个物种中，hisDNA在单个位点串联重复。基因组DNA的Southern印迹表明，这些簇代表了这些物种中绝大多数组蛋白基因。在这三个物种中发现了相似的重复值。仅在褐鳟的一个EcoRI位点发现了hisDNA的遗传变异。
