细胞外鸟苷和鸟苷-5'-三磷酸增加：培养的小鼠新皮质星形胶质细胞中神经生长因子的合成与释放。
Extracellular guanosine and guanosine-5'-triphosphate increase: NGF synthesis and release from cultured mouse neopallial astrocytes.
作者信息
Middlemiss P J, Gysbers J W, Rathbone M P
机构信息
Department of Biomedical Sciences, McMaster University, Hamilton, Ont., Canada.
出版信息
Brain Res. 1995 Apr 17;677(1):152-6. doi: 10.1016/0006-8993(95)00156-k.
Cultures of neonatal mouse cortical astrocytes synthesized NGF mRNA and released immunoreactive NGF (ir-NGF) into the culture medium. Addition of 10 microM guanosine or GTP to the cultures increased ir-NGF release by 6 and 2 fold, respectively, after 24 h, and increased NGF mRNA 6 fold after 4 h and 2-3 fold after 24 h. In contrast, neither adenosine nor ATP (each 1-100 microM) affected either NGF mRNA synthesis or ir-NGF release.
新生小鼠皮质星形胶质细胞培养物合成了神经生长因子（NGF）信使核糖核酸（mRNA），并将免疫反应性神经生长因子（ir-NGF）释放到培养基中。在培养物中添加10微摩尔的鸟苷或鸟苷三磷酸（GTP），24小时后ir-NGF的释放量分别增加了6倍和2倍，4小时后NGF mRNA增加了6倍，24小时后增加了2 - 3倍。相比之下，腺苷和三磷酸腺苷（ATP，均为1 - 100微摩尔）对NGF mRNA的合成或ir-NGF的释放均无影响。
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