Warbrick E V, Taylor A M, Botchkarev V A, Coleman J W
Department of Pharmacology and Therapeutics, University of Liverpool, United Kingdom.
Cell Immunol. 1995 Jul;163(2):222-8. doi: 10.1006/cimm.1995.1120.
We studied MHC class II gene expression and its regulation by IFN-gamma in purified rat peritoneal connective tissue-type mast cells. Mast cells were cultured with or without recombinant rat IFN-gamma (70 ng/ml) for 48 hr and analyzed by RT-PCR for expression of mRNA encoding MHC class II and by fluorescence flow cytometry for surface expression of MHC class II protein product. Levels of MHC class II mRNA and cell-surface protein product in untreated mast cells remained constant throughout the culture period but increased progressively after treatment with IFN-gamma such that by 48 hr levels were significantly greater than those in untreated cells. Dual labeling confirmed that MHC class II product was coexpressed with IgE (a mast cell marker). To conclude, rat connective tissue-type mast cells express mRNA and surface product for the MHC class II gene which can be up-regulated by IFN-gamma.
我们研究了纯化的大鼠腹膜结缔组织型肥大细胞中MHC II类基因的表达及其受γ干扰素的调控情况。肥大细胞在有或无重组大鼠γ干扰素（70 ng/ml）的条件下培养48小时，通过逆转录聚合酶链反应（RT-PCR）分析编码MHC II类的mRNA的表达情况，并通过荧光流式细胞术分析MHC II类蛋白产物的表面表达情况。未处理的肥大细胞中MHC II类mRNA和细胞表面蛋白产物的水平在整个培养期间保持恒定，但在用γ干扰素处理后逐渐增加，以至于到48小时时其水平显著高于未处理的细胞。双重标记证实MHC II类产物与IgE（一种肥大细胞标志物）共表达。总之，大鼠结缔组织型肥大细胞表达MHC II类基因的mRNA和表面产物，其可被γ干扰素上调。
