Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome.
作者信息
Kondoh Y, Ono T, Kagiyama N, Fujita S, Momiyama M, Hori S H, Yoshida M C
机构信息
Aisin Cosmos R&D Co., Ltd., Kariya, Aichi, Japan.
出版信息
Cytogenet Cell Genet. 1995;71(1):96-8. doi: 10.1159/000134071.
PMID:7606937
Abstract
We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.
摘要
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