Zhao L, Hayes K, Glassman A
Section of Clinical Cytogenetics, University of Texas, M. D. Anderson Cancer Center, Houston 77030, USA.
Cancer Genet Cytogenet. 1998 May;103(1):62-4. doi: 10.1016/s0165-4608(97)00345-2.
Several methods of chromosome identification to be used in combination with G-banding by the trypsin technique with Giemsa staining (G-banding) and fluorescence in situ hybridization (FISH) have been developed in the past few years. Unfortunately, these methods are impractical to use in the clinical laboratory and can provide inconsistent results. We report a simple, efficient, and reliable method of G-banding and FISH for use in clinical cytogenetics. G-banded chromosomes are codenatured with a direct- or indirect-labeled DNA prove (by using either chromosome painting or a chromosome centromeric probe) followed by a hybridization and signal detection. The procedure requires no pretreatment or additional fixation and results in good preservation of chromosome morphology and good intensity of the FISH signal.
在过去几年里，已经开发出了几种与胰蛋白酶技术吉姆萨染色法（G显带）和荧光原位杂交（FISH）相结合的染色体识别方法。不幸的是，这些方法在临床实验室中使用起来不切实际，并且可能会产生不一致的结果。我们报告了一种用于临床细胞遗传学的简单、高效且可靠的G显带和FISH方法。G显带染色体与直接或间接标记的DNA探针（通过使用染色体涂染或染色体着丝粒探针）进行共变性，随后进行杂交和信号检测。该程序无需预处理或额外固定，并且能很好地保存染色体形态以及获得强度良好的FISH信号。
