Vuillard L, Marret N, Rabilloud T
Institute Laue-Langevin, Grenoble, France.
Electrophoresis. 1995 Mar;16(3):295-7. doi: 10.1002/elps.1150160148.
A significant increase in the yield of protein extraction by the addition of sulfobetaine-type mild solubilization agents is shown for microsomal membrane proteins and proteins from lyophilized platelets, with a maximum increase in yield of up to 100%. These agents are also applied to native isoelectric focusing in immobilized pH gradients. The absence of significant denaturation induced by these solubilizers is shown for creatine kinase.
通过添加磺基甜菜碱型温和增溶剂，微粒体膜蛋白和冻干血小板中的蛋白质的蛋白质提取产率显著提高，产率最高可提高100%。这些试剂也应用于固定pH梯度的天然等电聚焦。对于肌酸激酶，显示出这些增溶剂不会引起明显的变性。
