Blisnick T, Morales-Betoulle M E, Vuillard L, Rabilloud T, Braun Breton C
Unit of Experimental Parasitology, Institut Pasteur, Paris, France.
Eur J Biochem. 1998 Mar 15;252(3):537-41. doi: 10.1046/j.1432-1327.1998.2520537.x.
A better understanding of the causative agent's biology and the definition of new targets for the development of drugs and/or specific immune responses is necessary to face the spred of drug-resistant malaria in developing countries and the absence of an efficient vaccine against this most important infectious disease. Non-detergent sulphobetaines enhance the recovery and isoelectric focussing of active Plasmodium falciparum proteases, cytoskeleton-associated proteins and Maurer's cleft-associated proteins. This is a significant advantage for the purification of such proteins and might help pinpoint their role for red blood cell rupture and merozoite release.
为应对耐药性疟疾在发展中国家的传播以及缺乏针对这种最重要传染病的有效疫苗的问题，有必要更好地了解病原体的生物学特性，并确定药物研发和/或特异性免疫反应的新靶点。非去污剂磺基甜菜碱可提高恶性疟原虫活性蛋白酶、细胞骨架相关蛋白和毛氏裂殖体相关蛋白的回收率和等电聚焦效果。这对于此类蛋白质的纯化而言是一个显著优势，可能有助于明确它们在红细胞破裂和裂殖子释放过程中的作用。
