Martínez J, Henriksson J, Rydåker M, Cazzulo J J, Pettersson U
Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina.
FEMS Microbiol Lett. 1995 Jun 15;129(2-3):135-41. doi: 10.1111/j.1574-6968.1995.tb07570.x.
PCR amplification of genomic DNA from the American trypanosome, Trypanosoma rangeli, using as primers oligonucleotides derived from the gene of cruzipain, the major cysteine proteinase (CP) from Trypanosoma cruzi, allowed the production of a probe which was used to obtain three clones encoding a CP with 70% overall identity with cruzipain. The genes are organized in tandem, with a monomere size of approximately 2 kbp, located on two chromosomes which, in some parasite isolates, have a high molecular mass (higher than 5.7 Mbp), and in others are much smaller (about 500 kbp). The low expression of this CP at the protein level correlates well with the low level of specific mRNA found in Northern blots.
使用源自克氏锥虫主要半胱氨酸蛋白酶（CP）——克鲁斯蛋白酶基因的寡核苷酸作为引物，对来自美洲锥虫——兰氏锥虫的基因组DNA进行PCR扩增，从而制备出一种探针，该探针用于获得三个编码与克鲁斯蛋白酶具有70%总体同一性的CP的克隆。这些基因串联排列，单体大小约为2 kbp，位于两条染色体上，在一些寄生虫分离株中，这两条染色体具有高分子量（高于5.7 Mbp），而在其他分离株中则小得多（约500 kbp）。这种CP在蛋白质水平上的低表达与Northern印迹中发现的特异性mRNA的低水平密切相关。
