Akif'ev A P, Khudoliĭ G A, Iakimenko A V, Krasnopevtsev A V, Khandogina E K
Genetika. 1995 Apr;31(4):485-91.
Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G1 phase (G1 process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G1 process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G1 process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G1 process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed.
本文提供了关于PHA刺激的处于G1期的人淋巴细胞中存在局部自发DNA合成（G1过程）的实验证据。该过程与单链DNA断裂的出现及其随后的修复相关。参与G1过程的DNA序列根据其核苷酸组成以及与核基质的关联进行了表征。结果表明，G1过程发生在辐射诱导交换型染色体畸变出现的染色体区域。该研究结果被视为支持染色体诱变初级接触理论分子版本的证据。文中还讨论了G1过程紊乱在人类染色体不稳定综合征发病机制中的可能作用。
