Dryden D T, Willcock D F, Murray N E
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Gene. 1995 May 19;157(1-2):123-4. doi: 10.1016/0378-1119(94)00630-b.
The EcoKI methyltransferase (M.EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.
EcoKI甲基转移酶（M.EcoKI，MTase）含有氨基酸（aa）序列AAGTA和NPPF，据信这两个序列在腺嘌呤甲基转移酶中高度保守[Klimasauskas等人，《核酸研究》17（1989）9823 - 9831]。我们分析了第一个序列中的一个突变，该突变消除了辅因子结合和酶活性，以及第二个序列中的突变，这些突变降低或消除了活性，但不影响辅因子和DNA结合。
