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低温下叶绿体735纳米荧光的寿命与产率之间的关系。

The relationship between the lifetime and yield of the 735 nm fluorescence of chloroplasts at low temperatures.

作者信息

Butler W L, Tredwell C J, Malkin R, Barber J

出版信息

Biochim Biophys Acta. 1979 Feb 8;545(2):309-15. doi: 10.1016/0005-2728(79)90208-1.

DOI:10.1016/0005-2728(79)90208-1
PMID:760782
Abstract

The lifetime and relative yield of the 735 nm fluorescence of chloroplasts, over a range of low temperatures (-60 to -196 degrees C) where the yield of fluorescence changes markedly, were found to be directly proportional. It is concluded that the species of chlorophyll responsible for the 735 nm fluorescence, C-705, is present over the entire temperature range but is less fluorescent at the higher temperatures because of greater energy transfer to P-700. It is also concluded from attempts to measure the rise-time of the 735 nm fluorescence at -196 degrees C that the rise-time is less than 50 ps.

摘要

在一系列低温(-60至-196摄氏度)范围内,叶绿体735纳米荧光的寿命和相对产率随荧光产率显著变化,结果发现二者成正比。得出的结论是,产生735纳米荧光的叶绿素种类C-705在整个温度范围内都存在,但在较高温度下荧光较弱,因为向P-700的能量转移更多。通过在-196摄氏度下测量735纳米荧光上升时间的尝试也得出结论,上升时间小于50皮秒。

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