乙醇对成年大鼠肝细胞原代单层培养物甘油脂质生物合成的影响。

The effect of ethanol on glycerolipid biosynthesis by primary monolayer cultures of adult rat hepatocytes.

作者信息

Wood C K, Lamb R G

出版信息

Biochim Biophys Acta. 1979 Jan 29;572(1):121-31. doi: 10.1016/0005-2760(79)90206-6.

Abstract

This study evaluates the effects of ethanol exposure on glycerolipid production and release by hepatocyte monolayers. Glycerolipid formation from [1,3-14C]glycerol was increased in monolayers exposed to ethanol (1--50 mM) for 6 h. Monolayers exposed to 1 mM ethanol for 24 h also exhibited a rise in glycerolipid formation from either [1,3-14C]glycerol or [1-14C]palmitate; however, higher ethanol concentrations produced a dose dependent decrease in glycerolipid formation. Glycerolipids released into the medium by monolayers declined after all periods of ethanol exposure. The effects of ethanol on the enzymatic reactions involved in glycerolipid biosynthesis were determined in homogenates prepared from monolayers exposed to ethanol. Two enzymes, phosphatidate phosphohydrolase and glycerol kinase, exhibited ethanol-induced alterations in enzyme activity; however, only glycerol kinase activity correlated well with monolayer glycerolipid formation. These ethanol-induced alterations in enzyme activities and glycerolipid biosynthesis were reduced by simultaneously exposing monolayers to pyrazole or cycloheximide.

摘要

本研究评估了乙醇暴露对单层肝细胞甘油脂质生成和释放的影响。暴露于乙醇（1-50 mM）6小时的单层肝细胞中，由[1,3-14C]甘油生成甘油脂质的量增加。暴露于1 mM乙醇24小时的单层肝细胞，由[1,3-14C]甘油或[1-14C]棕榈酸生成甘油脂质的量也有所增加；然而，较高浓度的乙醇会导致甘油脂质生成呈剂量依赖性下降。乙醇暴露各时间段后，单层肝细胞释放到培养基中的甘油脂质均减少。在由暴露于乙醇的单层肝细胞制备的匀浆中，测定了乙醇对甘油脂质生物合成中涉及的酶促反应的影响。两种酶，即磷脂酸磷酸水解酶和甘油激酶，表现出乙醇诱导的酶活性改变；然而，只有甘油激酶活性与单层肝细胞甘油脂质生成密切相关。通过同时将单层肝细胞暴露于吡唑或环己酰亚胺，可减少这些乙醇诱导的酶活性改变和甘油脂质生物合成。