Dich J, Bro B, Grunnet N, Jensen F, Kondrup J
Biochem J. 1983 Jun 15;212(3):617-23. doi: 10.1042/bj2120617.
Isolated hepatocytes from female rats were cultured in HI-WO/BA medium for 6 days. To the medium was added oleate, ethanol, dexamethasone and insulin. With oleate To alone, triacylglycerol accumulated; ethanol augmented the accumulation by 90%. To the best of our knowledge, this is the first demonstration that ethanol in vitro increases the content of triacylglycerol in liver cells. Further addition of dexamethasone or insulin did not alter the accumulation of triacylglycerol, indicating that these hormones did not play permissive roles for the effect of ethanol in the present system. Dexamethasone and insulin, in the absence of ethanol, increased the accumulation of triacylglycerol by 30% and 50% respectively. The concentration of glycerol 3-phosphate was increased in the presence of ethanol; however, with time the concentration of glycerol 3-phosphate declined almost to control values, while the accumulation of triacylglycerol continued linearly; this suggests that the effect of ethanol was not mediated via fluctuations in the concentration of glycerol 3-phosphate. These results are discussed in relation to earlier investigations in vivo and in vitro.
从雌性大鼠分离出的肝细胞在HI-WO/BA培养基中培养6天。向培养基中添加油酸、乙醇、地塞米松和胰岛素。仅添加油酸时，三酰甘油会积累；乙醇使积累量增加了90%。据我们所知，这是首次证明体外乙醇会增加肝细胞中三酰甘油的含量。进一步添加地塞米松或胰岛素并未改变三酰甘油的积累，这表明在本系统中这些激素对乙醇的作用没有起到允许作用。在没有乙醇的情况下，地塞米松和胰岛素分别使三酰甘油的积累增加了30%和50%。在乙醇存在的情况下，3-磷酸甘油的浓度升高；然而，随着时间推移，3-磷酸甘油的浓度几乎降至对照值，而三酰甘油的积累仍呈线性持续；这表明乙醇的作用不是通过3-磷酸甘油浓度的波动介导的。结合早期体内和体外研究对这些结果进行了讨论。
