Shiomi K, Kamiya H, Shimizu Y
Biochim Biophys Acta. 1979 Jan 25;576(1):118-27. doi: 10.1016/0005-2795(79)90490-2.
The red alga, Agardhiella tenera was found to contain a glycoprotein which agglutinates mouse leukemia cells, L5178Y but not L1210. It also agglutinates guinea pig and rabbit erythrocytes, and has weak activity against human A, B and O, mouse, horse and sheep erythrocytes and hamster and mouse lymphocytes. The agglutination was not inhibited by simple sugars. The major active component was purified and determined to be a beta-structure protein containing 2.7% glucose as sugar moiety. The molecular weight was estimated to be 12,000 by gel filtration and 13,000 by polyacrylamide gel electrophoresis, in the presence of sodium dodecylsulfate. Its isoelectric point was 6.1, and it contained high amounts of glycine, serine and threonine, but no half cystine or histidine. It had no subunit structure, and the C- and N-terminal amino acids were threonine and arginine, respectively.
人们发现红藻柔弱艾氏藻含有一种糖蛋白，它能凝集小鼠白血病细胞L5178Y，但不能凝集L1210。它还能凝集豚鼠和兔的红细胞，并且对人A、B、O型红细胞、小鼠、马和绵羊的红细胞以及仓鼠和小鼠淋巴细胞具有微弱活性。这种凝集作用不会被单糖抑制。主要活性成分被纯化，经测定是一种β结构蛋白，含有2.7%的葡萄糖作为糖基部分。在十二烷基硫酸钠存在的情况下，通过凝胶过滤法估计其分子量为12,000，通过聚丙烯酰胺凝胶电泳法估计为13,000。其等电点为6.1，含有大量的甘氨酸、丝氨酸和苏氨酸，但不含半胱氨酸或组氨酸。它没有亚基结构，C端和N端氨基酸分别是苏氨酸和精氨酸。
