Twagiramungu H, Guilbault L A, Proulx J G, Dufour J J
Département des Sciences Animales, Université Laval, Québec, Canada.
J Anim Sci. 1995 Mar;73(3):805-11. doi: 10.2527/1995.733805x.
Changes in proportions of luteal cells after buserelin treatment were studied in the corpus luteum (CL) that was present at the time of treatment (CLP) and in the buserelin-induced CL (CLI) of cyclic and acyclic postpartum cows. On d 0 of the experimental period, eight cyclic (Cyc-sal) cows were injected with saline, whereas eight cyclic (Cyc-bus) cows and eight acyclic (Acyc-bus) cows were treated i.m. with 8 micrograms of buserelin. On d 3 or 6, ovaries were collected and stained using histological techniques. As determined with the point-counting method, the number of nuclei in the CLP was similar on d 3 in Cyc-bus and Cyc-sal groups but was lower (treatment x day, P < .001) in the Cyc-bus group than in the Cyc-sal group on d 6. The volume of the CLP was similar on d 3 in the two groups but was greater (treatment x day, P < .04) in the Cyc-bus than in the Cyc-sal group on d 6. The number of nuclei in the CLI was greater (P < .001) in cyclic than in acyclic cows on d 3 and 6 after treatment. The volume of the CLI was lower (P < .001) in cyclic than in acyclic cows on d 3 and 6. Buserelin did not change the profile of progesterone in cyclic cows from d 0 to 6, but concentrations of progesterone increased (P < .01) in acyclic cows 3 to 6 d after buserelin treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了布舍瑞林治疗后黄体细胞比例的变化，对象为治疗时存在的黄体（CLP）以及产后周期性和非周期性奶牛的布舍瑞林诱导黄体（CLI）。在实验期第0天，8头周期性（Cyc-sal）奶牛注射生理盐水，而8头周期性（Cyc-bus）奶牛和8头非周期性（Acyc-bus）奶牛肌肉注射8微克布舍瑞林。在第3天或第6天，采集卵巢并使用组织学技术进行染色。通过点计数法测定，Cyc-bus组和Cyc-sal组在第3天时CLP中的细胞核数量相似，但在第6天时，Cyc-bus组的细胞核数量低于Cyc-sal组（处理×天数，P <.001）。两组在第3天时CLP的体积相似，但在第6天时，Cyc-bus组的CLP体积大于Cyc-sal组（处理×天数，P <.04）。治疗后第3天和第6天，周期性奶牛CLI中的细胞核数量多于非周期性奶牛（P <.001）。第3天和第6天，周期性奶牛CLI的体积低于非周期性奶牛（P <.001）。从第0天到第6天，布舍瑞林未改变周期性奶牛的孕酮水平，但在布舍瑞林治疗后3至6天，非周期性奶牛的孕酮浓度升高（P <.01）。（摘要截短至250字）
