Jones D P, Orrenius S, Mason H S
Biochim Biophys Acta. 1979 Jan 25;576(1):17-29. doi: 10.1016/0005-2795(79)90480-x.
Methods for quantitation of catalase, cytochromes P-450 and b5 and mitochondrial cytochromes a + a3, b561 + b566, and c + c1 in isolated hepatocytes were developed in analogy to methods established for subcellular systems and were used to measure changes in specific hemoprotein concentrations due to pretreatment and to change in incubation conditions. Pretreatment of rats with phenobarbital or 3-methylcholanthrene resulted in increased concentrations of cytochromes P-450 and b5 on a cellular basis, but had no effect on the other hemoproteins. Chronic ethanol pretreatment resulted in increased cytochrome P-450 and decreased cytochromes a + a3 concentrations. Hemoprotein concentrations in hepatocytes decreased following 4-10-h incubations in rotating round-bottom flasks. Rates of decrease were dependent upon both incubation conditions and prior in vivo treatments with phenobarbital or 3-methylcholanthrene.
针对分离的肝细胞中过氧化氢酶、细胞色素P-450和b5以及线粒体细胞色素a + a3、b561 + b566和c + c1的定量方法，是按照为亚细胞系统建立的方法类推而开发的，并用于测量由于预处理和孵育条件变化导致的特定血红蛋白浓度的变化。用苯巴比妥或3-甲基胆蒽对大鼠进行预处理，导致细胞基础上细胞色素P-450和b5的浓度增加，但对其他血红蛋白没有影响。慢性乙醇预处理导致细胞色素P-450增加，细胞色素a + a3浓度降低。在旋转圆底烧瓶中孵育4-10小时后，肝细胞中的血红蛋白浓度降低。降低速率取决于孵育条件以及先前用苯巴比妥或3-甲基胆蒽进行的体内处理。
