Grigolava I V, Konstantinov A A
Biokhimiia. 1979 Jul;44(7):1329-32.
The membrane-impermeable protein reagent p-diazonium benzene sulfonate (DABS) inhibits the mitochondrial respiratory chain in the cytochromes b-c1 region. It has been shown that DABS-treatment brings about a dramatic change in a low-temperature absorption spectrum of rat liver mictochondria, so that the long-wavelength alpha-band of ferrocytochrome b566 is sharply decreased, which is indicative of this hemoprotein modification. This observation suggest that cytochrome b566 is accessible to DABS at the outer surface of the coupling mitochondrial membrane.
膜不透性蛋白试剂对重氮苯磺酸盐（DABS）可抑制细胞色素b - c1区域的线粒体呼吸链。研究表明，用DABS处理会使大鼠肝脏线粒体的低温吸收光谱发生显著变化，亚铁细胞色素b566的长波长α带急剧下降，这表明该血红素蛋白发生了修饰。这一观察结果表明，细胞色素b566在偶联线粒体膜的外表面可被DABS作用。
