Brown E D, Vivas E I, Walsh C T, Kolter R
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
J Bacteriol. 1995 Jul;177(14):4194-7. doi: 10.1128/jb.177.14.4194-4197.1995.
The Escherichia coli gene murZ was recently shown to encode UDP-N-acetylglucosamine enolpyruvyl transferase, which catalyzes the first committed step of peptidoglycan biosynthesis (J. L. Marquardt, D. A. Siegele, R. Kolter, and C. T. Walsh, J. Bacteriol. 174:5748-5752, 1992). The map position of murZ (69.3 min) differed from that determined for murA (90 min), a gene which had been previously proposed to encode the same activity (P.S. Venkateswaran and H. C. Wu, J. Bacteriol. 110:935-944, 1972). Here we describe the construction of a chromosomal deletion of murZ and a plasmid containing murZ under arabinose control. Growth of cells containing the murZ deletion was dependent on the expression of murZ from the plasmid. We conclude that murZ is an essential gene and encodes the sole UDP-N-acetylglucosamine enolpyruvyl transferase of E. coli. To simplify the nomenclature, we recommend that murA be used to designate the gene at 69.3 min that encodes this activity and that the designation murZ be abandoned.
最近发现大肠杆菌基因murZ编码UDP-N-乙酰葡糖胺烯醇丙酮酸转移酶，该酶催化肽聚糖生物合成的第一步（J. L. Marquardt、D. A. Siegele、R. Kolter和C. T. Walsh，《细菌学杂志》174:5748 - 5752，1992年）。murZ的图谱位置（69.3分钟）与murA（90分钟）的不同，murA基因先前被认为编码相同的活性（P.S. Venkateswaran和H. C. Wu，《细菌学杂志》110:935 - 944，1972年）。在此我们描述了murZ染色体缺失体的构建以及一个在阿拉伯糖控制下含有murZ的质粒。含有murZ缺失体的细胞生长依赖于质粒上murZ的表达。我们得出结论，murZ是一个必需基因，并且编码大肠杆菌唯一的UDP-N-乙酰葡糖胺烯醇丙酮酸转移酶。为简化命名，我们建议用murA来指定位于69.3分钟处编码该活性的基因，而放弃murZ这一名称。
