Venkateswaran P S, Wu H C
J Bacteriol. 1972 Jun;110(3):935-44. doi: 10.1128/jb.110.3.935-944.1972.
A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of (3)H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.
从大肠杆菌K-12中分离出一个突变体，它对磷霉素表现出增强的抗性，磷霉素是最近从链霉菌菌株中分离出的一种新型杀菌抗生素。有证据表明，该突变体对磷霉素的裂解具有抗性，原因是磷酸烯醇丙酮酸：尿苷二磷酸-N-乙酰葡糖胺烯醇丙酮酸转移酶对这种抗生素的亲和力较低。还发现该突变体在生长方面对温度敏感。在42℃时，突变体细胞生长不良，并且（3）H-二氨基庚二酸掺入三氯乙酸不溶性物质的速率也大大降低。遗传学研究表明，该突变体对磷霉素抗性的增强和生长中对温度的敏感性可能是单个突变的结果。
