Licata A A, Au W Y, Vera J, Bartter F C
Biochim Biophys Acta. 1979 Jan 4;582(1):59-66. doi: 10.1016/0304-4165(79)90288-5.
We investigated some effects of prostaglandin E1 on the metabolism of rat parathyroid glands using a culture system containing basal Eagle's medium supplemented with 5--10% heat-inactivated rat serum. Rat parathyroid glands incorporate [3H]fucose and 14C-labeled amino acids into cellular glycoproteins and secrete some of these into the culture medium. Gel filtration chromatography separates these glycoproteins into three classes, the smallest of which (peak 3) is secreted with immunoreactive parathyroid hormone. In cultures of 48 h, prostaglandin E1 (1 microgram/ml) specifically inhibits the secretion of peak 3 and of parathyroid hormone but has no effect on the incorporation of [3H]fucose, 14C-labeled amino acids, or [3H]uridine into parathyroid glands. Cytochalasin B inhibits the secretion of parathyroid hormone and the incorporation of isotopic fucose and amino acids. Cortisol stimulates incorporation of [3H]fucose and the secretion of parathyroid hormone even in the presence of inhibitory doses of prostaglandin E1. It is concluded that, in organ culture, prostaglandin E1 inhibits the secretion of parathyroid hormone and of a specific glycoprotein the function of which may be related to the secretion of the hormone.
我们使用含有补充了5%-10%热灭活大鼠血清的基础伊格尔培养基的培养系统，研究了前列腺素E1对大鼠甲状旁腺代谢的一些影响。大鼠甲状旁腺将[3H]岩藻糖和14C标记的氨基酸掺入细胞糖蛋白中，并将其中一些分泌到培养基中。凝胶过滤色谱法将这些糖蛋白分为三类，其中最小的一类（峰3）与免疫反应性甲状旁腺激素一起分泌。在48小时的培养中，前列腺素E1（1微克/毫升）特异性抑制峰3和甲状旁腺激素的分泌，但对[3H]岩藻糖、14C标记的氨基酸或[3H]尿苷掺入甲状旁腺没有影响。细胞松弛素B抑制甲状旁腺激素的分泌以及同位素岩藻糖和氨基酸的掺入。即使在存在抑制剂量的前列腺素E1的情况下，皮质醇也会刺激[3H]岩藻糖的掺入和甲状旁腺激素的分泌。得出的结论是，在器官培养中，前列腺素E1抑制甲状旁腺激素和一种特定糖蛋白的分泌，该糖蛋白的功能可能与激素的分泌有关。
