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血小板膜离子化或可离子化基团的分析研究。

Analytical study of ionized or ionizable groups of platelet membrane.

作者信息

Stoltz J F, Nicolas A

出版信息

Blut. 1979 Feb 19;38(2):103-17. doi: 10.1007/BF01007951.

DOI:10.1007/BF01007951
PMID:760870
Abstract

The knowledge of the molecular surface groups of the human blood platelets present a particular interest especially in some thrombotic processes or thrombocytopathies. The aim of this study was to approach quantitatively ionized or ionizable groups of the platelet membrane using liquid phase electrophoresis or physicochemical and analytical methods with specific reagents. A mean repartition of the principal groups (carboxyl, SH, amino and phosphate) on the membrane is obtained by cell electrophoresis.

摘要

了解人类血小板的分子表面基团尤其在某些血栓形成过程或血小板病中具有特殊意义。本研究的目的是使用液相电泳或与特定试剂相关的物理化学和分析方法,对血小板膜的离子化或可离子化基团进行定量分析。通过细胞电泳获得了膜上主要基团(羧基、巯基、氨基和磷酸基团)的平均分布情况。

相似文献

1
Analytical study of ionized or ionizable groups of platelet membrane.血小板膜离子化或可离子化基团的分析研究。
Blut. 1979 Feb 19;38(2):103-17. doi: 10.1007/BF01007951.
2
Study of carboxyl groups on the human blood platelet membrane.人血小板膜上羧基的研究。
Biomedicine. 1977 Oct;27(7):271-3.
3
[Application of liquid phase electrophoresis to the study of phosphate groups in human platelets blocked by pralidoxime].[液相电泳在研究被解磷定阻断的人血小板中磷酸基团的应用]
C R Acad Hebd Seances Acad Sci D. 1974 Dec 16;279(25):1951-3.
4
Electrokinetic, analytical and functional heterogeneity of circulating human platelets: separation of subpopulations by continuous flow electrophoresis after taxol stabilization.
Biochim Biophys Acta. 1989 Oct 30;1014(1):26-39. doi: 10.1016/0167-4889(89)90237-1.
5
High resolution two-dimensional gel electrophoresis of the proteins and glycoproteins of human blood platelets and platelet membranes.人血小板及血小板膜蛋白质和糖蛋白的高分辨率二维凝胶电泳
Biochim Biophys Acta. 1979 May 3;553(1):11-24. doi: 10.1016/0005-2736(79)90027-0.
6
Isolation of human platelet and red blood cell plasma membrane proteins by preparative detergent electrophoresis.通过制备性去污剂电泳分离人血小板和红细胞质膜蛋白。
Biochim Biophys Acta. 1979 Jul 5;554(2):293-308. doi: 10.1016/0005-2736(79)90371-7.
7
Characterization of human blood platelet membrane proteins and glycorproteins by their isoelectric point (pI) and apparent molecular weight using two-dimensional electrophoresis and surface-labelling techniques.利用二维电泳和表面标记技术,通过等电点(pI)和表观分子量对人血小板膜蛋白和糖蛋白进行表征。
Biochim Biophys Acta. 1980 Jul;599(2):473-83. doi: 10.1016/0005-2736(80)90192-3.
8
Identification of human platelet membrane fibrinogen receptors by immunochemical techniques.通过免疫化学技术鉴定人血小板膜纤维蛋白原受体
Haematologia (Budap). 1984;17(3):387-98.
9
Distribution of free sulfhydryl and disulfide groups among platelet membrane proteins.
Biochim Biophys Acta. 1976 Jan 8;419(1):51-62. doi: 10.1016/0005-2736(76)90371-0.
10
[Membrane glycoprotein, glycocalicin, of human blood platelets].[人血小板的膜糖蛋白,糖钙蛋白]
Rinsho Byori. 1979;Suppl 40:231-43.

引用本文的文献

1
Topo-optical reactions of the human blood platelet membrane.人体血小板膜的拓扑光学反应。
Histochemistry. 1983;79(2):281-7. doi: 10.1007/BF00489790.
2
Glycocalyx electrostatic potential profile analysis: ion, pH, steric, and charge effects.糖萼静电势分布分析:离子、pH值、空间位阻及电荷效应
Yale J Biol Med. 1988 Sep-Oct;61(5):427-46.

本文引用的文献

1
[57] Bifunctional reagents.[57] 双功能试剂。
Methods Enzymol. 1972;25:623-51. doi: 10.1016/S0076-6879(72)25061-3.
2
[38] The rapid determination of amino groups with TNBS.[38] 用三硝基苯磺酸快速测定氨基。
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SIALIC ACID OF HUMAN BLOOD PLATELETS.人类血小板的唾液酸
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The contribution of sialic acid to the surface charge of the erythrocyte.唾液酸对红细胞表面电荷的贡献。
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The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
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A method for the quantitative modification and estimation of carboxylic acid groups in proteins.一种用于蛋白质中羧酸基团定量修饰和估算的方法。
J Biol Chem. 1967 May 25;242(10):2447-53.
7
Changes in the electrokinetic properties of platelets during their aggregation.血小板聚集过程中其电动特性的变化。
Arch Biochem Biophys. 1966 Oct;117(1):10-7. doi: 10.1016/0003-9861(66)90119-6.
8
The interaction of aldehydes with collagen.醛与胶原蛋白的相互作用。
Biochim Biophys Acta. 1968 Oct 21;168(2):341-52. doi: 10.1016/0005-2795(68)90156-6.
9
Inhibition of sulfhydryl-dependent platelet functions by penetrating and non-penetrating analogues of parachloromercuribenzene.
Blood. 1968 Apr;31(4):471-9.
10
Phosphate groups (receptors?) on the surface of human blood platelets.人类血小板表面的磷酸基团(受体?)
Nature. 1970 May 2;226(5244):452-3. doi: 10.1038/226452a0.