表达轮状病毒VP4区域、乙型肝炎表面抗原和单纯疱疹病毒2型糖蛋白D的重组脊髓灰质炎病毒的特性分析
Characterization of recombinant polioviruses expressing regions of rotavirus VP4, hepatitis B surface antigen, and herpes simplex virus type 2 glycoprotein D.
作者信息
Mattion N M, Reilly P A, Camposano E, Wu S L, DiMichele S J, Ishizaka S T, Fantini S E, Crowley J C, Weeks-Levy C
机构信息
Viral Vaccines Department, Lederle-Praxis Biologicals, Pearl River, New York 10965, USA.
出版信息
J Virol. 1995 Aug;69(8):5132-7. doi: 10.1128/JVI.69.8.5132-5137.1995.
Abstract
Recombinant polioviruses expressing antigens from rotavirus, herpes simplex virus type 2, and hepatitis B virus were generated. Fusion of the heterologous polypeptides to the amino terminus of the poliovirus polyprotein did not prevent myristylation of VP0, suggesting a novel mechanism of myristylation for these recombinant viruses. The effects of the parental genetic background, different foreign sequences, and different insert sizes on growth characteristics were compared. Both the size and the nature of the heterologous sequence appeared to be factors influencing the growth and stability of recombinant polioviruses. All of the recombinants showed a temperature-sensitive phenotype, regardless of the genetic background (attenuated or wild type) from which they were derived. Preliminary studies with transgenic mice carrying the poliovirus receptor gene are discussed.
摘要
产生了表达轮状病毒、单纯疱疹病毒2型和乙型肝炎病毒抗原的重组脊髓灰质炎病毒。将异源多肽融合到脊髓灰质炎病毒多聚蛋白的氨基末端并未阻止VP0的肉豆蔻酰化，这表明这些重组病毒存在一种新的肉豆蔻酰化机制。比较了亲本遗传背景、不同的外源序列和不同的插入大小对生长特性的影响。异源序列的大小和性质似乎都是影响重组脊髓灰质炎病毒生长和稳定性的因素。所有重组体均表现出温度敏感表型，无论其来源的遗传背景（减毒或野生型）如何。还讨论了对携带脊髓灰质炎病毒受体基因的转基因小鼠的初步研究。
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