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1
Interaction of nuclear protein p140 with human immunodeficiency virus type 1 TAR RNA in mitogen-activated primary human T lymphocytes.核蛋白p140与丝裂原激活的原代人T淋巴细胞中1型人类免疫缺陷病毒TAR RNA的相互作用
J Virol. 1995 Aug;69(8):5156-63. doi: 10.1128/JVI.69.8.5156-5163.1995.
2
A human chromosome 12-associated 83-kilodalton cellular protein specifically binds to the loop region of human immunodeficiency virus type 1 trans-activation response element RNA.一种与人类12号染色体相关的83千道尔顿细胞蛋白特异性结合人类免疫缺陷病毒1型反式激活应答元件RNA的环区。
J Virol. 1995 Oct;69(10):6593-9. doi: 10.1128/JVI.69.10.6593-6599.1995.
3
tat regulates binding of the human immunodeficiency virus trans-activating region RNA loop-binding protein TRP-185.反式激活转录物（tat）调节人类免疫缺陷病毒反式激活区RNA环结合蛋白TRP-185的结合。
Genes Dev. 1991 Nov;5(11):2128-40. doi: 10.1101/gad.5.11.2128.
4
Synthetic HIV-1 Tat can dissociate HeLa nuclear protein-TAR RNA complexes in vitro: a novel Tat-nuclear protein interaction.合成的HIV-1反式激活因子（Tat）能在体外解离HeLa细胞核蛋白-TAR RNA复合物：一种新型的Tat-核蛋白相互作用。
Oncogene. 1991 Sep;6(9):1507-13.
5
Molecular cloning and characterization of a TAR-binding nuclear factor from T cells.来自T细胞的一种TAR结合核因子的分子克隆与特性分析
AIDS Res Hum Retroviruses. 1995 Jun;11(6):663-9. doi: 10.1089/aid.1995.11.663.
6
Tat functions to stimulate the elongation properties of transcription complexes paused by the duplicated TAR RNA element of human immunodeficiency virus 2.Tat蛋白的功能是刺激因人类免疫缺陷病毒2的重复TAR RNA元件而暂停的转录复合物的延伸特性。
J Mol Biol. 1995 Dec 1;254(3):350-63. doi: 10.1006/jmbi.1995.0622.
7
Human immunodeficiency virus type 1 Tat-mediated trans activation correlates with the phosphorylation state of a cellular TAR RNA stem-binding factor.1型人类免疫缺陷病毒Tat介导的反式激活与一种细胞TAR RNA茎结合因子的磷酸化状态相关。
J Virol. 1992 Jul;66(7):4065-72. doi: 10.1128/JVI.66.7.4065-4072.1992.
8
Human immunodeficiency virus type 1 TAR element revertant viruses define RNA structures required for efficient viral gene expression and replication.1型人类免疫缺陷病毒TAR元件回复病毒确定了有效病毒基因表达和复制所需的RNA结构。
J Virol. 1995 Aug;69(8):4906-13. doi: 10.1128/JVI.69.8.4906-4913.1995.
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A bulge structure in HIV-1 TAR RNA is required for Tat binding and Tat-mediated trans-activation.HIV-1 TAR RNA中的一个凸起结构是Tat结合和Tat介导的反式激活所必需的。
Genes Dev. 1990 Aug;4(8):1365-73. doi: 10.1101/gad.4.8.1365.
10
Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells.细胞中Tat与人免疫缺陷病毒1型反式激活应答元件之间相互作用的功能分析。
J Virol. 1993 Sep;67(9):5617-22. doi: 10.1128/JVI.67.9.5617-5622.1993.

引用本文的文献

1
Recognition of 5'-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2.真核生物翻译起始因子2对人类免疫缺陷病毒1型mRNA 5'末端TAR结构的识别
Nucleic Acids Res. 2000 Feb 15;28(4):1011-8. doi: 10.1093/nar/28.4.1011.
2
The sequence and structure of the 3' arm of the first stem-loop of the human immunodeficiency virus type 2 trans-activation responsive region mediate Tat-2 transactivation.人类免疫缺陷病毒2型反式激活应答区域第一个茎环结构3'臂的序列和结构介导Tat-2反式激活。
J Virol. 1997 Oct;71(10):8048-55. doi: 10.1128/JVI.71.10.8048-8055.1997.
3
A human primary T-lymphocyte-derived human immunodeficiency virus type 1 Tat-associated kinase phosphorylates the C-terminal domain of RNA polymerase II and induces CAK activity.一种源自人原代T淋巴细胞的1型人类免疫缺陷病毒Tat相关激酶可使RNA聚合酶II的C末端结构域磷酸化并诱导CAK活性。
J Virol. 1997 Oct;71(10):7436-41. doi: 10.1128/JVI.71.10.7436-7441.1997.
4
Optimal Tat-mediated activation of the HIV-1 LTR promoter requires a full-length TAR RNA hairpin.HIV-1长末端重复序列（LTR）启动子的最佳Tat介导激活需要全长TAR RNA发夹结构。
Nucleic Acids Res. 1997 Feb 1;25(3):496-502. doi: 10.1093/nar/25.3.496.

本文引用的文献

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Mammalian G1 cyclins.哺乳动物G1期细胞周期蛋白。
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Effect of transforming growth factor-beta on early and late activation events in human T cells.转化生长因子-β对人T细胞早期和晚期激活事件的影响。
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Signal transduction by lymphocyte antigen receptors.淋巴细胞抗原受体的信号转导
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Transforming growth factor beta increases the expression of HIV-1 gene in transfected human mesangial cells.
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Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus.致肿瘤逆转录病毒需要经历有丝分裂，而人类免疫缺陷病毒则不需要。
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Regulation of human immunodeficiency virus infection: implications for pathogenesis.人类免疫缺陷病毒感染的调控：对发病机制的影响。
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A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells.HIV-1基质蛋白内的一个核定位信号，它控制非分裂细胞的感染。
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Human immunodeficiency virus type 1 DNA synthesis, integration, and efficient viral replication in growth-arrested T cells.1型人类免疫缺陷病毒在生长停滞的T细胞中的DNA合成、整合及高效病毒复制。
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Evolution of a disrupted TAR RNA hairpin structure in the HIV-1 virus.HIV-1病毒中TAR RNA发夹结构破坏的演变
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Signals and signs for lymphocyte responses.淋巴细胞反应的信号与体征
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核蛋白p140与丝裂原激活的原代人T淋巴细胞中1型人类免疫缺陷病毒TAR RNA的相互作用

Interaction of nuclear protein p140 with human immunodeficiency virus type 1 TAR RNA in mitogen-activated primary human T lymphocytes.

作者信息

Rothblum C J, Jackman J, Mikovits J, Shukla R R, Kumar A

机构信息

Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, D.C. 20037, USA.

出版信息

J Virol. 1995 Aug;69(8):5156-63. doi: 10.1128/JVI.69.8.5156-5163.1995.

DOI:10.1128/JVI.69.8.5156-5163.1995
PMID:7609087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189338/
Abstract

Several lines of evidence suggest that cellular proteins play a role during human immunodeficiency virus type 1 (HIV-1) Tat-mediated trans activation. A recent report from this laboratory has shown that a 140-kDa HeLa nuclear protein (p140) binds specifically to the lower stem region of the Tat response element, TAR RNA. Since HIV-1 trans activation is most efficient in proliferating T cells, we investigated the binding of p140 to TAR RNA in unstimulated and mitogen-activated, G1-phase primary T lymphocytes. TAR RNA/protein-binding activity was low in resting cells but increased significantly within 2 h of activation and remained elevated for at least 48 h. Corresponding increases in p140 protein levels were observed with most but not all donors, suggesting that an additional nuclear factor(s) may be required for efficient binding of this protein to TAR RNA in activated T cells.

摘要

多条证据表明，细胞蛋白在人类免疫缺陷病毒1型（HIV-1）Tat介导的反式激活过程中发挥作用。本实验室最近的一份报告显示，一种140 kDa的HeLa细胞核蛋白（p140）特异性结合Tat反应元件TAR RNA的下游茎区。由于HIV-1反式激活在增殖的T细胞中效率最高，我们研究了p140在未刺激的以及有丝分裂原激活的G1期原代T淋巴细胞中与TAR RNA的结合情况。TAR RNA/蛋白结合活性在静止细胞中较低，但在激活后2小时内显著增加，并至少持续升高48小时。在大多数但并非所有供体中都观察到p140蛋白水平相应增加，这表明在激活的T细胞中，该蛋白与TAR RNA的有效结合可能还需要其他核因子。