Howdieshell T R, Bhalla N, DiPiro J T, Kuske T, Baisden R
Department of Surgery, Medical College of Georgia, Augusta, USA.
JPEN J Parenter Enteral Nutr. 1995 Mar-Apr;19(2):125-6. doi: 10.1177/0148607195019002125.
Plasma triglyceride analyses to evaluate the clearance of IV fat emulsion are necessary in patients receiving parenteral nutrition. Enzymatic kits for triglyceride analysis measure the glycerol that is hydrolyzed from triglyceride. This study investigates the effect of fat emulsion free glycerol on plasma triglyceride determination.
Venous blood from fasting volunteers (n = 10) was drawn into tubes containing EDTA and was centrifuged to separate the plasma. An IV fat emulsion containing 2.25% glycerol was added to plasma samples to create serial dilutions ranging from 0.049% to 0.245% final lipid concentration. Total triglyceride and free glycerol concentrations were determined in each dilution and control sample. The free glycerol concentration was subtracted from the total triglyceride concentration to yield a true triglyceride value.
Increasing concentrations of fat emulsion added to donor plasma produced increases in total triglyceride and free glycerol concentrations. The increase in free glycerol concentration produced significant concentration-related differences between total and true triglyceride concentrations.
The total triglyceride level overestimated the true triglyceride concentration due to fat emulsion free glycerol. To assure reliable triglyceride results in patients receiving fat emulsion, blanking for free glycerol should be considered.
对于接受肠外营养的患者，进行血浆甘油三酯分析以评估静脉脂肪乳剂的清除情况是必要的。用于甘油三酯分析的酶试剂盒测量从甘油三酯水解而来的甘油。本研究调查了脂肪乳剂游离甘油对血浆甘油三酯测定的影响。
将来自空腹志愿者（n = 10）的静脉血采集到含有乙二胺四乙酸（EDTA）的试管中，离心分离出血浆。向血浆样本中加入含2.25%甘油的静脉脂肪乳剂，以产生最终脂质浓度范围为0.049%至0.245%的系列稀释液。测定每个稀释液和对照样本中的总甘油三酯和游离甘油浓度。从总甘油三酯浓度中减去游离甘油浓度以得出真实的甘油三酯值。
向供体血浆中添加的脂肪乳剂浓度增加，导致总甘油三酯和游离甘油浓度升高。游离甘油浓度的增加导致总甘油三酯浓度与真实甘油三酯浓度之间出现显著的浓度相关差异。
由于脂肪乳剂中的游离甘油，总甘油三酯水平高估了真实的甘油三酯浓度。为确保在接受脂肪乳剂的患者中获得可靠的甘油三酯结果，应考虑对游离甘油进行空白校正。
