Senatorov V V, Trudeau V L, Hu B
Neuroscience Unit, Loeb Research Institute, Ottawa Civic Hospital/University of Ottawa, Ont., Canada.
Brain Res Mol Brain Res. 1995 May;30(1):87-96. doi: 10.1016/0169-328x(94)00282-j.
Cholecystokinin (CCK), a well-known neuroactive peptide, has been observed in the axon endings within the thalamic reticular nucleus and the adjacent ventrolateral nucleus of the thalamus. The origin of this CCK innervation remains undefined. In this study, a fluorescence in situ hybridization (FISH) technique was used in conjunction with latex microsphere retrograde tracing to investigate whether cortical neurons may provide a source of CCK afferents to the ventrolateral thalamic nucleus. Rhodamine latex beads were injected into the ventrolateral thalamic nucleus of adult male rats to retrogradely label corticothalamic cells. After 7 days, tissues were processed for FISH using a 24-base oligonucleotide probe complementary to the 3' coding region of rat preprocholecystokinin mRNA. It was found that CCK transcripts are expressed in about 80% of identified corticothalamic projecting neurons. We therefore conclude that the descending cortical projections to the ventrolateral thalamus may provide an important source of CCK innervation to this region of the brain.
胆囊收缩素（CCK）是一种著名的神经活性肽，已在丘脑网状核和丘脑相邻腹外侧核内的轴突终末中观察到。这种CCK神经支配的起源尚不清楚。在本研究中，荧光原位杂交（FISH）技术与乳胶微球逆行追踪相结合，以研究皮质神经元是否可能为腹外侧丘脑核提供CCK传入神经的来源。将罗丹明乳胶珠注入成年雄性大鼠的腹外侧丘脑核，以逆行标记皮质丘脑细胞。7天后，使用与大鼠前胆囊收缩素原mRNA的3'编码区互补的24碱基寡核苷酸探针处理组织以进行FISH。发现约80%已鉴定的皮质丘脑投射神经元表达CCK转录本。因此，我们得出结论，下行至腹外侧丘脑的皮质投射可能为该脑区提供重要的CCK神经支配来源。
