Senatorov V V, Trudeau V L, Hu B
Loeb Research Institute, Ottawa Civic Hospital/University of Ottawa, Ontario, Canada.
Neuroscience. 1997 Aug;79(3):915-21. doi: 10.1016/s0306-4522(97)00045-6.
Cholecystokinin exerts a potent antiepileptic action in mammalian auditory system and undergoes seizure-mediated up-regulation. The present study investigated cholecystokinin messenger RNA expression in the reciprocally-connected auditory thalamus and cortex in the rat. Immunofluorescence in situ hybridization was performed using a 24-base cholecystokinin-messenger RNA oligonucleotide probe. Corticothalamic projection neurons were identified by means of the retrograde fluorescent tracer rhodamine latex microspheres injected into the medial geniculate body. In our experiments, cholecystokinin messenger RNA transcripts were found in about 80% of neurons located within the reciprocally-connected regions of the medial geniculate body and the auditory cortices. These observations provide evidence of cholecystokinin production in the reciprocally-connected regions of the auditory thalamus and cortex, the structures which jointly create the thalamo-corticothalamic circuit which has been implicated in seizure genesis.
胆囊收缩素在哺乳动物听觉系统中发挥强大的抗癫痫作用，并在癫痫发作时经历上调。本研究调查了大鼠相互连接的听觉丘脑和皮层中胆囊收缩素信使核糖核酸的表达。使用24个碱基的胆囊收缩素信使核糖核酸寡核苷酸探针进行免疫荧光原位杂交。通过将罗丹明乳胶微球逆行荧光示踪剂注入内侧膝状体来识别皮质丘脑投射神经元。在我们的实验中，在内侧膝状体和听觉皮层相互连接区域内约80%的神经元中发现了胆囊收缩素信使核糖核酸转录本。这些观察结果为在听觉丘脑和皮层的相互连接区域产生胆囊收缩素提供了证据，这些结构共同构成了与癫痫发作起源有关的丘脑 - 皮质丘脑回路。
