Kida S, Ellison D W, Steart P V, Weller R O
Department of Pathology (Neuropathology), Southampton General Hospital, UK.
Neuropathol Appl Neurobiol. 1995 Apr;21(2):121-9. doi: 10.1111/j.1365-2990.1995.tb01038.x.
Perivascular cells (PVCs) form an immunophenotypically defined population that plays an important scavenging role in the perivascular fluid drainage pathways in the rat brain; such cells may also act as antigen-presenting cells. The present study tests the hypotheses that (a) PVCs in human brain are distinct from microglia and haematogenous macrophages, and (b) PVCs within astrocytic tumours and peritumoral oedematous brain tissue react in a similar way to PVCs in the rat brain. Paraffin sections of formalin-fixed tissue from 10 astrocytomas, 10 anaplastic astrocytomas, 10 glioblastoma multiforme, peritumoral oedematous brain and from normal human brain were examined immunocytochemically using antibodies HLA-DR beta-chain for MHC class II antigen, PGM1 and MAC 387 directed against macrophage components, MT1 for T lymphocytes and GFAP for astrocytes. No PVCs, microglia or macrophages were labelled by these techniques in paraffin sections of normal brain. Microglia, macrophages recently derived from haematogenous monocytes and PVCs were labelled by immunocytochemistry in all tumours but were more numerous in glioblastomas than in astrocytomas or anaplastic astrocytomas. Perivascular cells were distinguished by their perivascular position, their expression of MHC class II antigen and were labelled by PGM1 antibody but not by MAC 387 antibody. Microglia and monocyte/macrophages, remote from blood vessels, on the other hand, were strongly labelled by MAC 387, moderately by PGM1 and showed weak expression of MHC class II antigen. A similar pattern of staining was seen in peritumoral oedematous tissue. These findings suggest that PVCs form a defined population of resident cells in the human brain and that they are distinct from microglia, monocytes and macrophages.(ABSTRACT TRUNCATED AT 250 WORDS)
血管周围细胞（PVCs）构成了一个免疫表型明确的细胞群体，在大鼠脑的血管周围液体引流途径中发挥着重要的清除作用；这类细胞也可能充当抗原呈递细胞。本研究检验以下假设：（a）人脑内的PVCs与小胶质细胞和血源性巨噬细胞不同；（b）星形细胞瘤和瘤周水肿脑组织内的PVCs与大鼠脑内的PVCs反应方式相似。使用针对MHC II类抗原的HLA - DRβ链抗体、针对巨噬细胞成分的PGM1和MAC 387抗体、针对T淋巴细胞的MT1抗体以及针对星形胶质细胞的GFAP抗体，对10例星形细胞瘤、10例间变性星形细胞瘤、10例多形性胶质母细胞瘤、瘤周水肿脑组织和正常人类脑组织的福尔马林固定组织石蜡切片进行免疫细胞化学检查。在正常脑的石蜡切片中，这些技术未标记出PVCs、小胶质细胞或巨噬细胞。通过免疫细胞化学在所有肿瘤中标记出了小胶质细胞、近期源自血源性单核细胞的巨噬细胞和PVCs，但在胶质母细胞瘤中比在星形细胞瘤或间变性星形细胞瘤中数量更多。血管周围细胞通过其血管周围位置、MHC II类抗原的表达得以区分，并且被PGM1抗体标记，但未被MAC 387抗体标记。另一方面，远离血管的小胶质细胞和单核细胞/巨噬细胞被MAC 387强烈标记，被PGM1中度标记，并显示出MHC II类抗原的弱表达。在瘤周水肿组织中也观察到了类似的染色模式。这些发现表明，PVCs在人脑中构成了一个明确的驻留细胞群体，并且它们与小胶质细胞、单核细胞和巨噬细胞不同。（摘要截短于250字）
