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编码小鼠Cdc21和CDC46同源物的cDNA的分子克隆及产物特性：P1（MCM3）与CDC46蛋白之间的物理相互作用

Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins.

作者信息

Kimura H, Takizawa N, Nozaki N, Sugimoto K

机构信息

Research Center for Molecular Genetics, Faculty of Science, Hokkaido University, Sapporo, Japan.

出版信息

Nucleic Acids Res. 1995 Jun 25;23(12):2097-104. doi: 10.1093/nar/23.12.2097.

DOI:10.1093/nar/23.12.2097
PMID:7610039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306996/
Abstract

Two new mouse genes encoding proteins that belong to the yeast minichromosome maintenance (MCM) protein family, which is involved in the initiation of DNA replication, were isolated and their nucleotide sequence was determined. They were a putative CDC46/MCM5 homolog and a putative cdc21 homolog. About 30% amino acid identity was obtained between members in the family, and > 40% between the putative mouse and yeast homologs. The expression of these genes was cell-cycle specific at the late G1 to S phase. Immunochemical analyses showed the physical interaction between mouse P1MCM3 and CDC46 protein. These results suggest that MCM proteins function in co-ordination for DNA replication.

摘要

分离出了两个新的小鼠基因，它们编码的蛋白质属于酵母微型染色体维持（MCM）蛋白家族，该家族参与DNA复制的起始，并确定了它们的核苷酸序列。它们分别是一个假定的CDC46/MCM5同源物和一个假定的cdc21同源物。该家族成员之间的氨基酸同一性约为30%，假定的小鼠和酵母同源物之间的氨基酸同一性大于40%。这些基因的表达在细胞周期的G1晚期到S期具有特异性。免疫化学分析显示小鼠P1MCM3和CDC46蛋白之间存在物理相互作用。这些结果表明MCM蛋白在DNA复制中协同发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/f059e5e9b353/nar00012-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/d153d6415fa9/nar00012-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/bc51cc884b4c/nar00012-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/a5144d6a38ec/nar00012-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/b97e74acde23/nar00012-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/f059e5e9b353/nar00012-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/d153d6415fa9/nar00012-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/bc51cc884b4c/nar00012-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/a5144d6a38ec/nar00012-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/b97e74acde23/nar00012-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc0f/306996/f059e5e9b353/nar00012-0033-b.jpg

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本文引用的文献

1
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Yeast. 1993 Jul;9(7):797-806. doi: 10.1002/yea.320090714.
2
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.防止DNA在单个细胞周期中再次复制：复制许可因子的证据。
J Cell Biol. 1993 Sep;122(5):993-1002. doi: 10.1083/jcb.122.5.993.
3
Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family.
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J Biol Chem. 2014 Oct 31;289(44):30810-30821. doi: 10.1074/jbc.M114.552935. Epub 2014 Sep 17.
4
Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication.禁止素与微小染色体维持蛋白物理相互作用并抑制哺乳动物DNA复制。
Cell Cycle. 2009 May 15;8(10):1621-9. doi: 10.4161/cc.8.10.8578. Epub 2009 May 27.
5
Eukaryotic MCM proteins: beyond replication initiation.真核生物微小染色体维持蛋白：超越复制起始
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6
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J Cell Biol. 2001 Jun 25;153(7):1341-53. doi: 10.1083/jcb.153.7.1341.
7
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Infect Immun. 2000 Oct;68(10):6069-72. doi: 10.1128/IAI.68.10.6069-6072.2000.
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4
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5
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8
Mutants of S. cerevisiae defective in the maintenance of minichromosomes.在小染色体维持方面存在缺陷的酿酒酵母突变体。
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9
A role for the nuclear envelope in controlling DNA replication within the cell cycle.核膜在细胞周期中控制DNA复制方面的作用。
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10
A mutant that affects the function of autonomously replicating sequences in yeast.一种影响酵母中自主复制序列功能的突变体。
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