Baskar L, Balasubramanian K A
Welcome Trust Research Laboratory, Dept. of Gastrointestinal Sciences, Christian Medical College Hospital, Vellore, India.
Scand J Gastroenterol. 1995 Apr;30(4):332-6. doi: 10.3109/00365529509093286.
The role of reactive oxygen species in injury to cells lining the gastrointestinal tract has gained importance in recent times, and their role in intestinal diseases has been studied.
In this study colonic epithelial cells isolated from the rat colon were exposed in vitro to various oxidants such as menadione, xanthine-xanthine oxidase, hydrogen peroxide, cumene hydroperoxide, and tertiary butyl hydroperoxide, separately. Changes in cell viability, thiol status, and the antioxidant enzyme activities were measured.
Colonocytes were found to be sensitive to menadione and were not affected by various other oxidants. Decrease in cell viability, depletion of reduced glutathione and protein thiol, and change in antioxidant enzyme activities were observed when the cells were exposed to menadione.
This study suggests that, unlike other cells, colonocytes are susceptible only to certain selective oxidants.
近年来，活性氧在胃肠道细胞损伤中的作用日益受到重视，并且其在肠道疾病中的作用也已得到研究。
在本研究中，从大鼠结肠分离出的结肠上皮细胞在体外分别暴露于各种氧化剂，如甲萘醌、黄嘌呤 - 黄嘌呤氧化酶、过氧化氢、氢过氧化异丙苯和叔丁基过氧化氢。测量细胞活力、巯基状态和抗氧化酶活性的变化。
发现结肠细胞对甲萘醌敏感，而不受其他各种氧化剂的影响。当细胞暴露于甲萘醌时，观察到细胞活力下降、还原型谷胱甘肽和蛋白质巯基减少以及抗氧化酶活性改变。
本研究表明，与其他细胞不同，结肠细胞仅对某些选择性氧化剂敏感。
