抗氧化剂缺乏的酿酒酵母中氧化剂诱导的鲁米诺发光。

Luminol luminescence induced by oxidants in antioxidant-deficient yeasts Saccharomyces cerevisiae.

作者信息

Jakubowski W, Ertel D, Biliński T, Kedziora J, Bartosz G

机构信息

Department of Molecular Biophysics, University of Lódź, Poland.

出版信息

Biochem Mol Biol Int. 1998 Jun;45(1):191-203. doi: 10.1080/15216549800202562.

DOI:10.1080/15216549800202562

PMID:9635143

Abstract

Luminol chemiluminescence induced in the presence of yeast cells and yeast cell homogenates was significantly induced by exogenous oxidants (hydrogen peroxide and menadione). tert-Butyl hydroperoxide did not stimulate chemiluminescence by itself but augmented menadione-induced chemiluminescence. Comparison of yeast strains deficient in catalase, superoxide dismutase or glutathione showed that only glutathione-deficient strains showed elevated chemiluminescence in this system. These results support the idea that more reactive species than hydrogen peroxide and superoxide are critical in the induction of luminol chemiluminescence.

摘要

在酵母细胞和酵母细胞匀浆存在的情况下，外源氧化剂（过氧化氢和甲萘醌）能显著诱导鲁米诺化学发光。叔丁基过氧化氢本身不会刺激化学发光，但会增强甲萘醌诱导的化学发光。对缺乏过氧化氢酶、超氧化物歧化酶或谷胱甘肽的酵母菌株进行比较后发现，在该系统中只有缺乏谷胱甘肽的菌株化学发光增强。这些结果支持了这样一种观点，即比过氧化氢和超氧化物更具反应活性的物质在鲁米诺化学发光的诱导中起关键作用。