Roa P N, Collins K A, Geisinger K R, Parsons L H, Schnell S, Hayworth-Hodge R, Tap M P, Lantz P E, Pettenati M J
Department of Pediatrics, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27157, USA.
Am J Clin Pathol. 1995 Jul;104(1):32-5. doi: 10.1093/ajcp/104.1.32.
In a prospective blinded controlled study, the efficacy of fluorescence in situ hybridization (FISH) to detect non-sperm male cells verifying sexual contact was examined. Cervicovaginal smears (CVS) from 40 women with reported post-coital intervals were examined for sperm by cytology and for sperm and non-sperm male cells by FISH using X and Y chromosome specific DNA probes. Fluorescence in situ hybridization identified sperm and/or non-sperm male cells in all specimens from women with positive coital histories, including when the partner had a vasectomy. Male cells were also detectable by FISH in CVS up to 3 weeks after coitus. In comparison, cytology identified sperm in 41% of the positive coital history cases, and none beyond 2 weeks. Fluorescence in situ hybridization is highly sensitive and specific in detecting male cells, and can be performed rapidly on routine CVS. Application of this technique can provide new and additional evidence of sexual contact when current tests are inconclusive.
在一项前瞻性双盲对照研究中，检测了荧光原位杂交（FISH）技术在检测证实性接触的非精子男性细胞方面的有效性。对40名有性交后间隔时间报告的女性的宫颈阴道涂片（CVS）进行了细胞学检查以检测精子，并使用X和Y染色体特异性DNA探针通过FISH检测精子和非精子男性细胞。荧光原位杂交在所有有阳性性交史的女性标本中均鉴定出精子和/或非精子男性细胞，包括其伴侣已进行输精管切除术的情况。性交后长达3周的CVS中，FISH也可检测到男性细胞。相比之下，细胞学在41%有阳性性交史的病例中鉴定出精子，且2周后均未检测到。荧光原位杂交在检测男性细胞方面具有高度敏感性和特异性，并且可以在常规CVS上快速进行。当当前检测结果不确定时，应用该技术可为性接触提供新的额外证据。
