Collins K A, Rao P N, Hayworth R, Schnell S, Tap M P, Lantz P E, Geisinger K R, Pettenati M J
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University.
J Forensic Sci. 1994 Nov;39(6):1347-55.
The identification of spermatozoa or constituents of seminal fluid is critical in the evaluation of alleged sexual assault victims. However, failure to identify sperm and/or elevated levels of acid phosphatase can occur for a variety of reasons. Molecular techniques, such as molecular cytogenetic analysis offers new approaches to improve on the identification of male cells in alleged sexual assault cases. Fluorescence in situ hybridization (FISH) with a Y chromosome specific DNA probe was applied to archival cervicovaginal smears from 41 alleged sexual assault cases to identify Y-bearing (male) cells. FISH identified Y-bearing sperm and non-sperm cells in 78% of the cases previously confirmed to have sperm. FISH also identified Y-bearing non-sperm male cells in 39% of the cases in which cytology did not detect spermatozoa; in one of these instances, it also detected sperm. Cervicovaginal acid phosphatase levels, determined at the time of the cervicovaginal smears, were also compared with the presence or absence of Y-positive cells. Application of this technique can detect non-spermatozoic male cells in routine cervicovaginal smears of sexual assault victims.
精液中精子或精液成分的鉴定对于涉嫌性侵犯受害者的评估至关重要。然而，由于多种原因，可能无法鉴定出精子和/或酸性磷酸酶水平升高。分子技术，如分子细胞遗传学分析，为改进涉嫌性侵犯案件中男性细胞的鉴定提供了新方法。使用Y染色体特异性DNA探针的荧光原位杂交（FISH）技术应用于41例涉嫌性侵犯案件的存档宫颈阴道涂片，以鉴定含Y染色体（男性）的细胞。FISH在先前已确认有精子的78%的病例中鉴定出含Y染色体的精子和非精子细胞。FISH在细胞学未检测到精子的39%的病例中也鉴定出含Y染色体的非精子男性细胞；在其中一个病例中，还检测到了精子。在进行宫颈阴道涂片时测定的宫颈阴道酸性磷酸酶水平也与Y阳性细胞的存在与否进行了比较。应用该技术可在性侵犯受害者的常规宫颈阴道涂片中检测到非精子男性细胞。
