Mattana J, Margiloff L, Singhal P C
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, NY 11040, USA.
Biochem Biophys Res Commun. 1995 Jul 6;212(1):63-9. doi: 10.1006/bbrc.1995.1936.
Oxygen radicals are believed to play a role in the pathogenesis of aging and glomerular injury. Changes in cellular function may result from modification of the extracellular matrix with which they interact. We produced oxidized mesangial matrix protein using a mixed function oxidase system. Carbonyl content of the oxidized matrix was significantly increased. Adhesion of macrophages to the oxidized mesangial matrix was significantly enhanced, an effect which was significantly abrogated by scavenger receptor blockade with polyinosinic acid or dextran sulfate. Neither polyinosinic acid nor dextran sulfate diminished macrophage adhesion to unmodified matrix. These data demonstrate for the first time that mesangial matrix can undergo oxidation and that such oxidation may promote accumulation of macrophages in the mesangium via interaction of macrophage scavenger receptors with oxidized matrix proteins.
氧自由基被认为在衰老和肾小球损伤的发病机制中起作用。细胞功能的变化可能源于它们与之相互作用的细胞外基质的修饰。我们使用混合功能氧化酶系统产生了氧化的系膜基质蛋白。氧化基质的羰基含量显著增加。巨噬细胞与氧化的系膜基质的粘附显著增强，用聚肌苷酸或硫酸葡聚糖阻断清道夫受体可显著消除这种作用。聚肌苷酸和硫酸葡聚糖均未减少巨噬细胞与未修饰基质的粘附。这些数据首次证明系膜基质可发生氧化，并且这种氧化可能通过巨噬细胞清道夫受体与氧化基质蛋白的相互作用促进巨噬细胞在系膜中的积聚。
